. 2021 May 10;8:653148. doi: 10.3389/fmolb.2021.653148

TABLE 1.

SCoV2 protein constructs expressed and purified, given with the genomic position and corresponding PDBs for construct design.

Protein genome position (nt) ^a	Trivial name construct expressed	Size (aa)	Boundaries	MW (kDa)	Homol. SCoV (%) ^b	Template PDB ^c	SCoV2 PDB ^d
nsp1	Leader	180		19.8	84
266–805	Leader	180		19.8	84
	Full-length	180	1–180	19.8	83
	Globular domain (GD)	116	13–127	12.7	85	2GDT	7K7P
nsp2		638		70.5	68
806–2,719		638		70.5	68
	C-terminal IDR (CtDR)	45	557–601	4.9	55
nsp3		1,945		217.3	76
2,720–8,554		1,945		217.3	76
a	Ub-like (Ubl) domain	111	1–111	12.4	79	2IDY	7KAG
a	Ub-like (Ubl) domain + IDR	206	1–206	23.2	58
b	Macrodomain	170	207–376	18.3	74	6VXS	6VXS
c	SUD-N	140	409–548	15.5	69	2W2G
c	SUD-NM	267	409–675	29.6	74	2W2G
c	SUD-M	125	551–675	14.2	82	2W2G
c	SUD-MC	195	551–743	21.9	79	2KQV
c	SUD-C	64	680–743	7.4	73	2KAF
d	Papain-like protease PL^pro	318	743–1,060	36	83	6W9C	6W9C
e	NAB	116	1,088–1,203	13.4	87	2K87
Y	CoV-Y	308	1,638–1,945	34	89
nsp5	Main protease (M^pro)	306		33.7	96
10,055–10,972	Main protease (M^pro)	306		33.7	96
	Full-length ^e	306	1–306	33.7	96	6Y84	6Y84
nsp7		83		9.2	99
11,843–12,091		83		9.2	99
	Full-length	83	1–83	9.2	99	6WIQ	6WIQ
nsp8		198		21.9	98
12,092–12,685		198		21.9	98
	Full-length	198	1–198	21.9	97	6WIQ	6WIQ
nsp9		113		12.4	97
12,686–13,024		113		12.4	97
	Full-length	113	1–113	12.4	97	6W4B	6W4B
nsp10		139		14.8	97
13,025–13,441		139		14.8	97
	Full-length	139	1–139	14.8	97	6W4H	6W4H
nsp13	Helicase	601		66.9	100
16,237–18,039	Helicase	601		66.9	100
	Full-length	601	1–601	66.9	100	6ZSL	6ZSL
nsp14	Exonuclease/methyltransferase	527		59.8	95
18,040–19,620	Exonuclease/methyltransferase	527		59.8	95
	Full-length	527	1–527	59.8	95	5NFY
	MTase domain	240	288–527	27.5	95
nsp15	Endonuclease	346		38.8	89
19,621–20,658	Endonuclease	346		38.8	89
	Full-length	346	1–346	38.8	89	6W01	6W01
nsp16	Methyltransferase	298		33.3	93
20,659–21,552	Methyltransferase	298		33.3	93
	Full-length	298	1–298	33.3	93	6W4H	6W4H
ORF3a		275		31.3	72
25,393–26,220		275		31.3	72
	Full-length	275	1–275	31.3	72	6XDC	6XDC
ORF4	Envelope (E) protein	75		8.4	95
26,245–26,472	Envelope (E) protein	75		8.4	95
	Full-length	75	1–75	8.4	95	5X29	7K3G
ORF5	Membrane glycoprotein (M)	222		25.1	91
26,523–27,387	Membrane glycoprotein (M)	222		25.1	91
	Full-length	222	1–222	25.1	91
ORF6		61		7.3	69
27,202–27,387		61		7.3	69
	Full-length	61	1–61	7.3	69
ORF7a		121		13.7	85
27,394–27,759		121		13.7	85
	Ectodomain (ED)	66	16–81	7.4	85	1XAK	6W37
ORF7b		43		5.2	85
27,756–27,887		43		5.2	85
	Full-length	43	1–43	5.2	85
ORF8		121		13.8	32
27,894–28,259		121		13.8	32
ORF8	Full-length	121	1–121	13.8	32
ΔORF8	w/o signal peptide	106	16–121	12	41	7JTL	7JTL
ORF9a	Nucleocapsid (N)	419		45.6	91
28,274–29,533	Nucleocapsid (N)	419		45.6	91
	IDR1-NTD-IDR2	248	1–248	26.5	90
	NTD-SR	169	44–212	18.1	92
	NTD	136	44–180	14.9	93	6YI3	6YI3
	CTD	118	247–364	13.3	96	2JW8	7C22
ORF9b		97		10.8	72
28,284–28,574		97		10.8	72
	Full-length	97	1–97	10.8	72	6Z4U	6Z4U
ORF14		73		8	n.a
28,734–28,952		73		8	n.a
	Full-length	73	1–73	8	n.a
ORF10		38		4.4	29
29,558–29,674		38		4.4	29
	Full-length	38	1–38	4.4	29

^{^a}

Genome position in nt corresponding to SCoV2 NCBI reference genome entry NC_045512.2, identical to GenBank entry MN908947.3.

^{^b}

Sequence identities to SCoV are calculated from an alignment with corresponding protein sequences based on the genome sequence of NCBI Reference NC_004718.3.

^{^c}

Representative PDB that was available at the beginning of construct design, either SCoV or SCoV2.

^{^d}

Representative PDB available for SCoV2 (as of December 2020).

^{^e}

Additional point mutations in fl-construct have been expressed.

n.a.: not applicable.