Table 1.
Composition of the WE- and CAS-based diets fed to male Sprague Dawley rats for 2 weeksa.
| Ingredient (g/kg) | CAS | WE |
|---|---|---|
| Casein | 200 | – |
| Whole eggb | – | 435 |
| Cornstarch | 417 | 365 |
| Glucose monohydrate | 150 | 150 |
| Corn oil | 183 | – |
| Mineral mix | 35 | 35 |
| Vitamin mix | 10 | 10 |
| Choline bitartrate | 2 | 2 |
| L-methionine | 3 | 3 |
| Biotin (1%) | – | 0.4 |
| Macronutrients (% total kcal)c | ||
| Protein | 17 | 17 |
| Carbohydrate | 48 | 48 |
| Fat | 35 | 35 |
| Caloric content | 4,715 | 4,715 |
All ingredients were purchased from Envigo except for dried whole egg (Rose Acre Farms, Guthrie Center, IA), as well as L-methionine and choline bitartrate (Sigma-Aldrich). CAS, casein-based diet, WE, whole egg-based diet.
Total protein and lipid content provided by 435 g of dried whole egg was 46 (200 g) and 42% (183 g), respectively.
All diets formulated to contain total protein at 20% (w/w).