Table 1.

Comparing the performance of cell-free expression systems⁴⁹⁾

Materials	E. coli	Rabbit reticulocyte	Wheat embryos
Productivity (per ml)	6 mg	µg order	9.7 mg
N-terminala	N-formylmethionine	Mature	Mature
Folding	Post-translation	Co-translation	Co-translation
Quality	Low	High	High
Codon preference	Tight	Tight	Loose
Amino acid-specific isotopic labeling	Difficult	—	Yes
Post-translational modification	No	Yes	Yes
Membrane protein	Yes	Yes	Yes
Disulfide-bond formation	Yes	—	Yes

^aN-terminal methionine is processed by the endogenous N-terminal methionine peptidase.^57,58)