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. 2021 May 10;11:637823. doi: 10.3389/fonc.2021.637823

Table 4.

Pre-specified analysis of the VELOUR trial.

Analysis Objectives Variables Results Reference
Pre-specified subgroup analysis Assessment of treatment effect in specific subgroups Demographic characteristics: No significant treatment interaction between FOLFIRI-aflibercept vs FOLFIRI-placebo and factors for both OS and PFS (107)
Age <65/≥65 years Treatment effect favored aflibercept over control (HR: <1.0) for OS and PFS in all subgroups
Male/female
Caucasian-white/other
Western Europe/Eastern Europe/North America/South America/Other countries
Baseline characteristics: Consistent treatment effect in favor of the aflibercept for OS and PFS for all subgroups
Prior/no prior hypertension Significantly greater aflibercept benefit in case of liver-only metastases vs no liver metastases or liver and other sites metastases: p=0.090 for OS; p=0.008 for PFS
Number of organs with metastasis ≤1/>1
No liver metastasis or liver and other
metastasis/liver metastasis only
Colon-rectosigmoid-other/rectum
Stratification factors: A difference in favor of aflibercept over placebo in OS and PFS in each stratification subgroup; no significant interaction at the two-sided 10% level between treatment and stratification levels → no evidence of heterogeneity in treatment effect
ECOG PS ECOG PS 0 vs 1 vs 2: p=0.7231 for OS; p=0.6954 for PFS
Prior bevacizumab Prior bevacizumab (30.4% of ITT) vs no prior bevacizumab: p=0.5668 for OS; p=0.1958 for PFS
FOLFIRI-aflibercept vs control:
mOS
Prior bevacizumab: 12.5 m vs 11.7 m
No prior bevacizumab: 13.9 m vs 12.4 m
mPFS
Prior bevacizumab: 6.7 m vs 3.9 m
No prior bevacizumab: 6.9 m vs 5.4 m
No evidence of greater toxicity in pts previously treated with bevacizumab
Pre-specified post hoc multivariate analysis of the ITT population - Identification of prognostic factors associated with improved OS with FOLFIRI-aflibercept Better efficacy subgroup: Better efficacy subgroup FOLFIRI-aflibercept vs FOLFIRI-placebo: (108)
-Primary endpoint: OS in the better and poorer efficacy patient subgroup identified in the multivariate analysis ECOG PS 0 with any number of metastatic site or ECOG PS 1 with <2 metastatic sites Interaction with treatment: p = 0.0147 → differential OS effect of aflibercept compared with placebo
Poorer efficacy subgroup: mOS 16.2 m (95% CI: 14.5–18.1) vs 13.1 m (95% CI: 11.7–14.2) (absolute difference in mOS: 3.1 m; aHR: 0.73; 95%CI: 0.61–0.86)
ECOG PS 1 with ≥2 metastatic sites or ECOG PS 2 with any number of metastatic sites or ARR (excluded from the analysis) Continuous increase over time of the entity of survival differences between the two treatment arms: absolute OS rate difference 5% at 6 m →15% at 30 m
mPFS 7.2 m (95%CI: 6.8–8.2) vs 4.8 m (95%CI: 4.2–5.4)(absolute difference: 2.4 m)
Absolute difference in 6-month PFS rates: 25%
ORR 23.7% (95% CI: 19.3–28.2) vs 11% (95% CI: 7.8–14.3)
Poorer efficacy subgroup FOLFIRI-aflibercept vs FOLFIRI-placebo:
No OS, PFS and ORR improvement with FOLFIRI-aflibercept vs FOLFIRI-placebo
mOS
9.6 m (95% CI: 8.6–11.5) vs
10.4 m (95% CI: 9.5–12.1)
(aHR: 0.97; 95% CI: 0.78–1.21)
Aged-based analysis Assessment of benefit and safety of aflibercept in association with FOLFIRI according to age Age ≥65 y No treatment interaction between treatment group and age was reported for OS (p=0.683) or PFS (p=0.930) (109)
(36%; 84% aged 65–74 y, 97% ECOG PS 0–1) ≥65 y FOLFIRI-aflibercept vs FOLFIRI-placebo:
Age <65 y (64%) mOS 12.6 m vs 11.3 m
(HR: 0.85; 95.34%CI: 0.68–1.07), absolute difference: 1.3 m
mPFS 6.6 m vs 4.4 m
(HR: 0.75; 99.99% CI: 0.48–1.17), absolute difference: 2.2 m
<65 y FOLFIRI-aflibercept vs FOLFIRI-placebo:
mOS 14.5 m vs 12.5 m
(HR: 0.80; 95.34%CI: 0.67–0.95)
mPFS 6.9 m vs 4.9 m
(HR: 0.77; 99.99%CI: 0.55–1.08), absolute difference 2 m

AEs, adverse events; aHR, adjusted HR; CI, confidence interval; ECOG PS, Eastern Cooperative Oncology Group Performance Status; FOLFIRI, fluorouracil, levofolinic acid, irinotecan; G, grade; HR, hazard ratio, ITT, intent to treat; m, months; mOS, median overall survival; mPFS, median progression free survival; ORR, objective response rate; OS, overall survival; PFS, progression free survival; y, years.

The underlined text and the bold values refer to some points that we would like to emphasize, e.g. endpoints, treatment arms.