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. 2021 May 10;12:667242. doi: 10.3389/fpls.2021.667242

FIGURE 1.

FIGURE 1

Sequence analysis of the GmGAMYB. (A) Multiple sequence alignment. Multiple sequence alignment of soybean GmGAMYB and GAMYB-like proteins of Arabidopsis thaliana (At), Oryza sativa (Os), Zea mays (Zm), Vitis vinifera (Vv), Manihot esculenta (Me), Solanum lycopersicum (Sl), and Cucumis sativus (Cs) (accession numbers are listed in Supplementary Table 1). The conserved R2R3-type domain is underlined. (B) Conservative Motif distribution of all GmGAMYB and GAMYB-Kike proteins using the MEME Suite, and ten motifs were identified. The sequences of the ten motifs are exhibited at the bottom. (C) Phylogenetic tree analysis was performed on GmGAMYB and proteins from other species with high similarity in NCBI. In addition to nine GAMYB-like proteins in the eight species mentioned above, Mucuna pruriens (Mp), Spatholobus suberectus (Ss), Cajanus cajan (Cc), Vigna unguiculata (Vu), Vigna angularis (Va), Medicago truncatula (Mt), Lupinus angustifolius (La), Populus trichocarpa (Ptr), Ricinus communis (Rc), Hordeum vulgare (Hv) were added to the construction of the phylogenetic tree. All the amino acid sequence information comes from the Phytozome database (accession numbers are listed in Supplementary Table 1). Phylogenetic tree was constructed using the neighbor joining method of MEGA 6.0.