Figure 1.

Bioinformatic identification of an archaeal cardiolipin synthase (Cls).A, hydropathy profile alignment of E. coli ClsA (red line), with the averaged hydropathy profile of its bacterial protein family (black line). B, schematic representation of potential membrane topologies of cardiolipin synthase with two predicted transmembrane anchors and a globular active domain. C, unrooted tree of putative archaeal cardiolipin synthases with two main clusters. # E. coli ClsA; ∗ M. hungatei Cls. A detailed tree can be found in the Fig. S1. D, consensus sequence logo of the second hydrophobic region of cardiolipin synthase type A enzymes from bacteria and the group of methanogenic archaea.