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. 2021 Apr 22;296:100691. doi: 10.1016/j.jbc.2021.100691

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MhCls activity in the presence or absence of glycerol starting with the substrate(s).A, Di-oleoyl-phosphatidylglycerol (DOPG), (B) glycerol-di-phosphatidyl-cardiolipin (Gro-DPCL) 18:1/18:1/18:1/18:1, and (C) palmitoyl-oleoyl-phosphatidylglycerol (POPG) together with Gro-DPCL 18:1/18:1/18:1/18:1. The formed lipid species PG, Gro-DPCL, and phosphatidic acid (PA) were analyzed by LC-MS, normalized for the internal standard DDM, and ion counts are plotted on the y-axis. Lines represent the mean of the data points (n = 2). D, schematic representation of the MhCls-mediated glycerol-dependent dynamic equilibrium in Gro-DPCL formation.