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. 2021 Apr 22;296:100691. doi: 10.1016/j.jbc.2021.100691

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

MhCls-dependent glyco-mono-phosphatidyl-cardiolipin formation.A, structures of glycolipid mono-galactosyl-diacylglycerol (MGDG) and 1-galactosyl-mono-phosphatidyl-cardiolipin (1Gal-MPCL). B, MhCls-mediated formation of glycerol-di-phosphatidyl-cardiolipin (Gro-DPCL), di-oleoyl-phosphatidic acid (DOPA), and 1Gal-MPCL from the substrates MGDG and di-oleoyl-phosphatidylglycerol (PG). Lipid species were analyzed by LC-MS, normalized for the internal standard DDM, and plotted. Data are mean ± SD (n = 3). Statistical significance is shown for the enzymatic reaction (MhCls) compared with the control (no enzyme), for each individual lipid species, by using the Student’s t-test analysis; ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001; ∗∗∗∗p ≤ 0.0001.