. 2021 Apr 22;296:100691. doi: 10.1016/j.jbc.2021.100691

Table 1.

Cloning and expression vectors used in this study

Plasmid	Description	Reference
pRSF-Duet-1	Expression vector (Kan^R), T7 promoter	Novagen
pNDK001	MhCls gene with N-terminus 6x His-tag from M. hungatei JF-1 cloned into pRSF-Duet vector using the primers NDKo027 and NDKo028.	This study
pBAD	Expression vector, pBR322 ori; araC; pBAD, Zeo^R	This study
pME006	MhCls gene with N-terminus 6x His-tag from M. hungatei JF-1 cloned into pBAD-vector using the primers prME010 and prME011 to amplify the MhCls gene, and primers prME012 and prME013 to amplify the pBAD backbone.	This study