Table 2.
Primers | Primer sequence 5’ -> 3’ | Restriction site |
---|---|---|
NDKo027 | ACAGTTCCATGGCCCATCACCATCATCACCACATCCATGATCTGATTCTGGTGATCCACAATTTTC | NcoI |
NDKo028 | ACTTACGAGCTCTTATTATTACAGCAGCGGACTAAACAGACG | SacI |
prME010 | TATCGAATTCATGCATCACCATCATCACCACATCCATGATCTGA | EcoRI |
prME011 | CTATAAGCTTTTATTATTACAGCAGCGGACTAAACAGACGGCTAAT | HindIII |
prME012 | ACTCGAATTCTTCCTCCTAGCCTGCTTTTTTGT | EcoRI |
prME013 | ACTGAAGCTTGATATCGTTTAAACGGTCTCCAGCTTGG | HindIII |
Introduced restriction sites are underlined.