Fig. 2.

Loss of Setd2 in IECs aggravates DSS-induced colitis in mice.

(A) Immunohistochemical analyses of SETD2 and H3K36me3 expressions are shown.

(B) Immunoblot analyses of SETD2 and H3K36me3 expression in IECs from Setd2^Vil-KO and Setd2^f/f mice are shown. Scale Bars: 20 μm.

(C) Schematic representation of the DSS protocol used to induce acute colitis in Setd2^Vil-KO and Setd2^f/f mice.

(D, E) Setd2^Vil-KO and Setd2^f/f mice were fed 2% DSS in drinking water, and loss of body weights (D) and colon length (E) are recorded (n = 6 per genotype). Scale Bars: 1 cm.

(F) H&E stained sections of colon tissues collected on day 0 and 10 from 2% DSS-treated Setd2^Vil-KO and Setd2^f/f mice, and the quantitation of DSS-induced epithelial damage and immune cell infiltration are shown in the right (n = 6 per genotype). Scale Bars: 50 μm.

(G) Alcian blue-Periodic acid Schiff (AB-PAS; goblet cells) staining in the colon and lysozyme (Lys; Paneth cells) staining in the small intestine as indicated (n = 6 per genotype). Scale Bars: 20 μm.

(H) After 5 d of DSS treatment, colonic lamina propria cells from Setd2^Vil-KO and Setd2^f/fmice are analyzed by flow cytometry for CD4 + T cells, CD11b +; F4/80 + macrophages, and CD11b +; Gr-1 + neutrophils (n = 3 per genotype).

(I) Colon sections from mice treated with DSS were stained for F4/80 to detect macrophages. Scale Bars: 50 μm.

(J) Relative mRNA expression levels of inflammatory mediators in whole colon of Setd2^Vil-KO and Setd2^f/f mice were determined by RT-qPCR (n = 6 per genotype).

The data represent the mean ± S.E.M, and statistical significance was determined by a two-tailed Student's t-test. *p < 0.05, **p < 0.01 and ***p < 0.001. N.S., Not Significant. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)