Table 1.

Comparison of sample input, reagents, and time required for standard versus rapid protocols for fungi

Organism	Input	Formaldehyde per sample	DNA purification method	Days for protocola
S. cerevisiae Standardb	200 mL liquid culture	5.5 mL	Phenol/chloroform extraction & ethanol precipitation	3
S. cerevisiae Rapid	20 OD∗mL pellet (from 25 mL culture)	27 μL	MinElute column	1.5
S. pombe Standardc	100 mL liquid culture	2.7 mL	Phenol/chloroform extraction & ethanol precipitation	3
S. pombe Rapid	20 OD∗mL pellet (from 25 mL culture)	27 μL	MinElute column	3
N. crassa Standard Ad	Isolated nuclei from 500 mL conidia	None	MinElute column	2
N. crassa Standard Be	Isolated chromatin fraction from 50 mL conidia	275 μL	Phenol/chloroform extraction & ethanol precipitation	3
N. crassa Rapid	50 mL conidia	1.4 mL	MinElute column	1

^aNumber of days required to isolate mononucleosomal fragments from starting material (input)

^bRodriguez et al, 2014

^cCam and Whitehall, 2016

^dKlocko et al, 2019

^eSeymour et al, 2016