Supplementary Figure 1.

The best intervention method and concentration of losartan in the treatment of OA mice. A, Degree of cartilage damage of knee joint after treatment with different concentrations of losartan assessed by safranin-O-fast green staining (400 ​× ​). B, OARSI scores of mice after treatment with different concentrations of losartan. C and D, Expression of PPARγ, TGF-β1, Smad2, and Smad3, along with the extent of Smad2 and Smad3 phosphorylation in OA mice after treatment with different concentrations of losartan evaluated by Western blot analysis normalized to GAPDH. E and F, Expression of MMP-13, ADAMTS-4, ADAMTS-5, HtrA1, iNOS, and Collagen II in mice after treatment with different concentrations of losartan evaluated by Western blot analysis normalized to GAPDH. G and H, The levels of IL-6, IL-1β, TNF-α, and COX-2 in mice after treatment with different concentrations of losartan evaluated by ELISA. ∗p ​< ​0.05 vs. OA mice treated with normal saline; #p ​< ​0.05 vs. OA mice treated with 0.1 ​mg/mL or 0.1 ​mg/kg losartan; & p ​< ​0.05 vs. OA mice treated with 1 ​mg/mL or 1 ​mg/kg losartan.