Figure 2.

Remote ischemic conditioning decreases the infarct volume and improves behavioral function. (a) Representative TTC staining images of different sections of brain tissues from two groups (6 mice per group) were shown. The numbers 1 to 6 represented coronal sections at different planes of each mouse brain. The brain tissue with pale TTC staining was the infarct region. (b) Total infarct volume (%) for six sections of each brain was evaluated by TTC staining in the different groups, and compared between the two groups by the Student’s t-test. (c) Infarct volume (%) by TTC staining in each of the six sections was compared by the Student’s t-test. Infarct volume was calculated using the following formula: [(volume of nonischemic hemisphere) − (volume of nonischemic region of ischemic hemisphere)/2 × volume of nonischemic hemisphere × 100%]. (d) Neurological function was assessed by the Longa scoring system in the different groups, and compared by the non-parametric test (Mann–Whitney test). (e) Delay time in stick strip removal during the adhesive removal test was compared between the groups by the Student’s t-test. *p < 0.05.**p < 0.01. The findings were similar when the same experiment was repeated independently.