Figure 6.

Comparison of the transcriptional changes in different IS etiologies versus VRFC in monocytes and neutrophils. Venn diagrams for the genes that meet the defined criteria (FC > |1.2|, p < 0.05 for the individual contrasts and having Sample_Type * Diagnosis FDR (step up) < 0.2). From the unique genes included in the Venn diagrams (not overlapped regions), the corresponding PCA is shown for (a) monocytes and (c) neutrophils. The effects of additional factors were removed before plotting. Ellipsoids represent 2 standard deviations from the centroid. Venn diagrams of the numbers of over-represented (Fisher’s p-value < 0.05) canonical pathways in (b) monocytes and (d) neutrophils. Top 10 over-represented canonical pathways exclusively found in CE, LV, and SV stroke in (e) monocytes and (f) neutrophils. In the plot, orange bars indicate predicted activation of the pathway, blue ones show predicted inhibition, white bars – no direction can be predicted, and grey depicts no prediction can be performed. The shades for every colored cell represent z-scores values. Arrows indicate predicted significant activation (Z ≥ 2, up arrow) or significant inhibition (Z ≤ −2, down arrow) of the pathway. The vertical orange line marks the −log(p-value)>1.3 threshold. In neutrophils, superpathway of geranylgeranyl diphosphate biosynthesis i (via mevalonate) and regulation (reg) are abbreviated.