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. Author manuscript; available in PMC: 2021 May 24.
Published in final edited form as: Cell Rep. 2021 Apr 20;35(3):109009. doi: 10.1016/j.celrep.2021.109009

Figure 2. LUAD cells at either end of the EMT spectrum shape tCAF heterogeneity.

Figure 2.

(A) GSEA of upregulated genes in tCAFs that were mono- or co-cultured (left), co-cultured with 344SQ cells or 393P cells (center), or co-cultured with ZEB1-deficient (shZEB1) or ZEB1-replete (control shRNA [shCTL]) 344SQ cells (right) in multicellular aggregates. NES represented by bar length. FDR values color-coded. Hallmarks shared between co-cultures are indicated (asterisks).

(B) PCA plot of tCAFs from Boyden chamber preparations (dots) subjected to bulk-cell RNA sequencing. GSEA of upregulated genes in tCAFs that were mono-cultured (mono-) or co-cultured (co-) with 393P cells or 344SQ cells in Boyden chambers. NES represented by bar length. FDR values color-coded. n = 5 biological replicates per condition.