Table 1. The SRX state of myosin measured by mant-ATP/ATP chase assays in skinned PMs from the hearts of HCM-A57G, RCM-E143K, Δ43, and WT-ELC mice.
| Parameter | WT | HCM-A57G | Δ43 | RCM-E143K |
|---|---|---|---|---|
| P1 (%) | 55.8 ± 3.8 | 67.4 ± 8.4a | 48.5 ± 9.5b | 48.3 ± 8.4c |
| P2 (%) | 41.5 ± 3.8 | 29.5 ± 8.4a | 49.4 ± 8.7b | 49.6 ± 7.4c |
| T1 (s) | 8.2 ± 4.3 | 6.2 ± 3.4 | 4.9 ± 3.5 | 9.0 ± 5.2 |
| T2 (s) | 182.8 ± 126.3 | 109.0 ± 71.5 | 197.3 ± 108.7 | 186.3 ± 123.9 |
| Number of animals | 11 (5 M, 6 F) | 8 (4 M, 4 F) | 10 (5 M, 5 F) | 7 (4 M, 3 F) |
Data are mean ± SD. P1 and P2 represent the percentage of myosin cross-bridges in the DRX and SRX states, respectively; T1 and T2 depict their respective lifetimes. P values were calculated with one-way ANOVA with Tukey’s multiple comparison test.
a
P < 0.05 for mutant versus WT-ELC.
b
P < 0.05 for Δ43 versus A57G.
c
P < 0.05 for E143K versus A57G.