Figure 2.

Depletion of circ_0013359 inhibits proliferation, migration, invasion, and glycolysis and induces apoptosis and cycle arrest in melanoma cells. A875 and SK-MEL-1 cells were introduced with si-NC or si-circ_0013359. Cell viability (a and b), colony numbers (c), apoptosis (d), and cell cycle distribution (e and f) were detected by MTT assay, colony formation assay, and flow cytometry, respectively. (g and h) Transwell assay was used to assess cell migration and invasion. (i and j) Glucose consumption and lactate production were measured after transfection for 48 h. (k and l) Seahorse XF^e 96 Extracellular Flux Analyzer was used to detect ECAR. (m and n) The levels of HK2 and LDHA were tested using western blot. *P < 0.05.