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. 2021 May 22;16(1):482–494. doi: 10.1515/biol-2021-0030

Figure 3.

Figure 3

Circ_0013359 directly interacts with miR-136-5p. (a and b) The levels of the selected six targets (miR-145-5p, miR-136-5p, miR-331-3p, miR-370-3p, miR-637, and miR-767-5p) were detected in A875 and SK-MEL-1 cells transfected with si-NC or si-circ_0013359. (c and d) The levels of miR-136-5p and circ_0013359 were measured in A875 and SK-MEL-1 cells transfected with miR-NC or miR-136-5p. (e) The predicted binding site between circ_0013359 and miR-136-5p was exhibited. (f and g) The luciferase activity was tested in A875 and SK-MEL-1 cells co-transfected with circ_0013359 WT or circ_0013359 MUT and miR-NC or miR-136-5p. (h and i) RIP assay was used to confirm the binding relationship between circ_0013359 and miR-136-5p. (j) MiR-136-5p level was detected in melanoma tissues and normal tissues. (k) Expression of miR-136-5p was examined in HEMn-LP, A875, and SK-MEL-1 cells. *P < 0.05.