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. 2021 May 22;16(1):482–494. doi: 10.1515/biol-2021-0030

Figure 5.

Figure 5

MiR-136-5p directly targets RAB9A. (a) Venn diagram showing seven possible target genes for miR-136-5p in four databases (Starbase, Targetscan, miRDB, and TarBase v.8). (b and c) The expression of seven possible target genes was detected in A875 and SK-MEL-1 cells transfected with miR-NC or miR-136-5p. (d) The putative binding sequence between miR-136-5p and RAB9A 3′-UTR was displayed. (e and f) Dual-luciferase reporter assay was used to verify the relationship between miR-136-5p and RAB9A. (g and h) The mRNA and protein levels of RAB9A were tested in melanoma tissues and normal tissues. (i and j) The expression of RAB9A in HEMn-LP, A875, and SK-MEL-1 cells was measured by qRT-PCR and western blot. (k) RAB9A protein level was determined in A875 and SK-MEL-1 cells transfected with miR-NC or miR-136-5p. (l and m) The mRNA and protein levels of RAB9A were examined in A875 and SK-MEL-1 cells introduced with si-NC, si-circ_0013359, si-circ_0013359 + anti-miR-NC, or si-circ_0013359 + anti-miR-136-5p. *P < 0.05.