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Compatibility of decalcification agents with staining methods
| Compatibility of staining | |||||
| Decalcification | H&E | TRAP | Toluidine blue | Safranin O | IHC |
| EDTA | Highly | Highly | Highly | Less35 | Highly |
| Formic acid | Highly | Less* | Highly | Highly | Not compatible with some epitopes31 36 38 |
*Expert experiences.
IHC, immunohistochemistry; TRAP, tartrate-resistant acid phosphatase.
