TABLE 1.
Characteristics and accession numbers for genomes of the apple stigma bacterial isolates
| Characteristic | Data for: |
||||
|---|---|---|---|---|---|
| Curtobacterium sp. 24E2 | Erwinia sp. 18B1 | Pantoea sp. 1B4 | Pseudomonas sp. 15A4 | Pseudomonas sp. 1079 | |
| Mean raw sequence length (bp) | 7,304 | 6,911 | 6,430 | 7,384 | 6,627 |
| Total sequences (Gb) | 2.17 | 2.05 | 2.10 | 1.37 | 0.57 |
| Mean coverage (×) | 555 | 384 | 391 | 229 | 91 |
| Flow cell barcode sequences | TCCATTCCCTCCGATAGATGAAAC | ACGTAACTTGGTTTGTTCCCTGAA | AAGGATTCATTCCCACGGTAACAC | TAGGGAAACACGATAGAATCCGAA | a |
| Total sequence length (bp) | 3,805,409 | 5,153,201 | 5,091,746 | 5,702,273 | 5,898,439 |
| No. of scaffolds | 1 | 5 | 6 | 1 | 2 |
| Raw read N50 (bp) | 15,616 | 13,014 | 12,702 | 15,569 | 14,902 |
| Assembly N50 (bp) | 3,805,409 | 4,898,912 | 4,115,195 | 5,702,273 | 5,894,865 |
| GC content (%) | 70.7 | 56.4 | 55.1 | 60.5 | 60.2 |
| No. of genes | 3,743 | 5,039 | 4,823 | 5,039 | 5,343 |
| No. of protein-coding genes | 3,043 | 3,661 | 3,711 | 4,318 | 4,389 |
| No. of 16S rRNAs | 4 | 7 | 7 | 5 | 6 |
| No. of tRNAs | 47 | 81 | 77 | 64 | 68 |
| Completeness (%)b | 87.33 | 87.18 | 96.23 | 98.57 | 97.27 |
| Contamination (%)b | 5.08 | 6.90 | 0.45 | 0.03 | 0.27 |
| Closest relative (ANI [%], 16S rRNA gene identity [%]) | Curtobacterium citreum (85.5, 90.75) | Erwinia aphidicola (98.7, 95.00) | Pantoea agglomerans (98.3, 99.87) | Pseudomonas graminis (91.7, 99.93) | Pseudomonas carnis (98.5, NAc) |
| Genome GenBank accession no. | CP068987 | JAEUXA000000000 | JAEUWZ000000000 | CP068986 | JAEUXB000000000 |
| Raw read SRA no. | SRR13499755–SRR13499826 | SRR13499599–SRR13499670 | SRR13499518–SRR13499596 | SRR13500215–SRR13500259 | SRR13499671–SRR13499754 |
a
This isolate was sequenced on a Flongle flow cell, while the other strains were barcoded and sequenced on an R9.4 flow cell.
b
Based on marker gene analysis in the CheckM software package.
c
NA, no available 16S rRNA gene sequence in the NCBI database.