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ZO-1 staining for BRB integrity. The representative images display ZO-1 staining in the RPE layer at day 3 after rAION induction. The ZO-1 was labeled with FITC (green), and the nuclei were stained with DAPI (blue). Scale bar: 50 µm. BRB, blood-retinal barrier; DAPI, 4, 6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; rAION, rodent model of anterior ischemic optic neuropathy; RPE, retinal pigment epithelium; ZO-1, zona occludens 1.
