Table 3.

Xeno- and Feeder-Free Differentiation by Recombinant Cytokines

Stage	Medium	Serum	High Receptors	Cytokines	Concentration	Differentiation Period
1	MEM-α	KnockOut Serum Replacement	TGFBR1	TGF-β1	2 ng/mL	7 d
			PTPRN	NGF-β	100 ng/mL
			EPOR	Erythropoietin	2 U/mL
2	MEM-α	KnockOut Serum Replacement	TGFBR2	TGF-β1	2 ng/mL	14 d
			TGFBR3
			PTPRN	NGF-β	100 ng/mL
			EPOR	Erythropoietin	2 U/mL
			PTGER	PGF2α	10 µM
			PTGFR
			MEGF9	EGF	10 ng/mL
			EDIL3