Figure 1.

Effect of anti-spike antibodies on ACE2 binding

(A) HEK293T cells transfected with vectors expressing NTD-TM, RBD-TM, and S2-TM were stained with anti-spike antibodies. The effect of antibodies on ACE2-binding to spike transfectants was analyzed with ACE2-Fc-fusion protein.

(B) Mean fluorescence intensities (MFIs) of the stained cells are shown (top three columns). The binding of ACE2-Fc-fusion protein to full-length spike transfectants was analyzed in the presence of the indicated antibodies at 1 μg/mL (bottom column). Antibodies that enhanced binding of ACE2-Fc to the spike transfectants by more than 1.9 times are indicated in red.

See also Figures S1 and S2.