Figure 3.

Enhanced SARS-CoV-2 infectivity by specific anti-NTD antibodies.

(A) ACE2-expressing HEK293T cells (MOI: 0.3) were infected by a SARS-CoV-2 spike pseudovirus carrying a GFP reporter gene in the presence of various concentrations of indicated antibodies. The proportion of GFP-positive infected cells is shown.

(B) ACE2-expressing HEK293T cells were infected with a SARS-CoV-2 spike pseudovirus carrying a GFP reporter gene at different MOIs with (red line, 3 μg/mL) or without (black line) the indicated antibodies.

(C) HEK293T cells, ACE2-expressing HEK293T cells, and Huh7 cells were infected with authentic SARS-CoV-2 virus in the presence (+) or absence (−) of enhancing antibody COV2-2490 at 1 μg/mL. The amounts of SARS-CoV-2 virus produced in the cell culture supernatants were analyzed 48 h after infection. The statistical significance derived from an unpaired t test is indicated. NS, not significant. The data are presented as mean ± SD. Representative data from three independent experiments are shown.

See also Figure S1.