Figure S5.
Induction of the open conformation of the RBD by divalent enhancing antibodies, related to Figure 6
(A) The binding of antibodies specific to open RBD (H014) or open and closed RBD (C009, C144, C135) against spike transfectants was analyzed in the presence or absence of enhancing (COV2-2490) or non-enhancing (4A8) antibodies (3 ug/ml).
(B) SDS-PAGE analysis of whole IgG antibody, F(ab’)2 and Fab fragments of enhancing COV2-2490 antibody.
(C) Whole spike transfectants were stained with whole IgG antibody, F(ab’)2 or Fab fragment of COV2-2490 antibody. Bound antibodies were detected by APC-labeled anti-human IgG Fc or anti-human IgG Fab specific antibodies.