Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 24;184(13):3438–3451.e10. doi: 10.1016/j.cell.2021.05.031

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Elsevier Inc.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Binding between ACE2 orthologs and the SARS-CoV-2 RBD or RaTG13 RBD

(A) Flow cytometry analysis of binding between 25 ACE2 orthologs and the SARS-CoV-2 RBD or RaTG13 RBD. HEK293T cells expressing EGFP-fused ACE2s are stained with the indicated His-tagged proteins (RaTG13 RBD, SARS-CoV-2 RBD, and MERS-CoV RBD). An anti-His/Allophycocyanin (APC) antibody is used to detect His-tagged proteins. The MERS-CoV RBD is used as a negative control.

(B) SPR characterizations of the binding between 25 ACE2 orthologs and the RaTG13 RBD. ACE2s with a mouse Fc (mFc) tag are immobilized on a CM5 chip. SPR characterizations of the binding affinity between the RaTG13 RBD and each ACE2 ortholog are shown. PD-L1 is used as a negative control. Raw and fitted curves are displayed as blue and red lines, respectively. Data represent the mean ± SD of three independent experiments.

See also Figures S2 and S3 and Tables S1 and S2.