REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial strains
Escherichia coli (E. coli) strain DH5α	TIANGEN	Cat# CB101-02
Escherichia coli (E. coli) strain BL21 (DE3)	Novagen	Cat# 69450
MAX Efficiency DH10Bac Competent E. coli	Invitrogen	Cat# 10361-012
Chemicals, antibodies, and recombinant proteins
PEI	Alfa	A04043896-1g
Anti-His/APC	Miltenyi Biotec	Cat# 130-119-820; RRID: AB_2751870
SARS-CoV-2 RBD protein with his-tag, spike residues 319-541, accession number: EPI_ISL_402119	This paper	N/A
Goat anti-human IgG-HRP	Thermo Fisher	Cat# 31410
SARS-CoV-2 RBD-F449Y	This paper	N/A
SARS-CoV-2 RBD-L486F	This paper	N/A
SARS-CoV-2 RBD-Q493Y	This paper	N/A
SARS-CoV-2 RBD-Q498Y	This paper	N/A
SARS-CoV-2 RBD-D501N	This paper	N/A
SARS-CoV-2 RBD-H505Y	This paper	N/A
SARS-CoV RBD protein with his-tag, spike residues 306-527, accession number: NC_004718	This paper	N/A
cACE2 protein, residues 18-740, accession number: NP_001034545.1	This paper	N/A
Critical commercial assays
HisTrap HP 5 mL column	GE Healthcare	Cat# 17524802
HiLoad 16/600 Superdex 200 pg	GE Healthcare	Cat# 28989335
Series S Sensor Chip CM5	GE Healthcare	Cat# 29149603
Sensor Chip Protein A	GE Healthcare	Cat# 29127556
Membrane concentrator	Millipore	UFC901096
Deposited data
RaTG13 RBD/hACE2 complex	This paper	Protein Data Bank: 7DRV
Experimental models: Cell lines
Sf9 Cells, SFM Adapted	Invitrogen	Cat# 11496015
High Five cells	Invitrogen	Cat# B85502
HEK293T cells	ATCC	ATCC CRL-3216
Recombinant DNA
pEGFP-N1	MiaoLingPlasmid	Cat# P0133
pEGFP-N1-hACE2, accession number: BAJ21180	This paper	N/A
pEGFP-N1-monkey ACE2, accession number: A0A2K5X283	This paper	N/A
pEGFP-N1-rabbit ACE2, accession number: G1TEF4	This paper	N/A
pEGFP-N1-mouse ACE2, accession number: Q8R0I0	This paper	N/A
pEGFP-N1-rat ACE2, accession number: Q5EGZ1	This paper	N/A
pEGFP-N1-malayan pangolin ACE2, accession number: XP_017505746	This paper	N/A
pEGFP-N1-catACE2, accession number: Q56H28	This paper	N/A
pEGFP-N1-civet ACE2, accession number: Q56NL1.1	This paper	N/A
pEGFP-N1-fox ACE2, accession number: XP_025842512.1	This paper	N/A
pEGFP-N1-dog ACE2, accession number: J9P7Y2	This paper	N/A
pEGFP-N1-raccoon dog ACE2, accession number: ABW16956.1	This paper	N/A
pEGFP-N1-horse ACE2, accession number: F6V9L3	This paper	N/A
pEGFP-N1-pig ACE2, accession number: A0A220QT48	This paper	N/A
pEGFP-N1-wild Bactrian camel ACE2, accession number: XP_006194263.1	This paper	N/A
pEGFP-N1-alpaca ACE2, accession number: XP_006212709.1	This paper	N/A
pEGFP-N1-bovine ACE2, accession number: Q58DD0	This paper	N/A
pEGFP-N1-goat ACE2, accession number: XP_005701129.2	This paper	N/A
pEGFP-N1-sheep ACE2, accession number: W5PSB6	This paper	N/A
pEGFP-N1-intermediate horseshoe bat ACE2, accession number: W5PSB6	This paper	N/A
pEGFP-N1-Least horseshoe bat ACE2, accession number: W5PSB6	This paper	N/A
pEGFP-N1-little brown bat ACE2, accession number: G1PXH7	This paper	N/A
pEGFP-N1-fulvous fruit bat ACE2, accession number: D8WU01	This paper	N/A
pEGFP-N1-greater horseshoe bat ACE2, accession number: B6ZGN7	This paper	N/A
pEGFP-N1-big-eared horseshoe bat ACE2, accession number: XP_007538670.1	This paper	N/A
pEGFP-N1-lesser hedgehog tenrec ACE2, accession number: XP_004710002.1	This paper	N/A
pFastbac1	Invitrogen	10360014
pFastbac-SARS-CoV-2 RBD -His, residues 319-541, accession number: EPI_ISL_402119	This paper	N/A
pFastbac- SARS-CoV RBD -His, residues 306-527, accession number: NC_004718	This paper	N/A
pCAGGS	MiaoLingPlasmid	Cat# P0165
pET21a	Novagen	Cat# 69740
pET21a-hACE2, residues 18-740, accession number: NP_001034545.1	This paper	N/A
Software and algorithms
PyMOL software	Molecular Graphics System, Version 1.8 Schrödinger	https://pymol.org/2/
BIAcore 8K Evaluation software	GE Healthcare	N/A
FlowJo V10	FLOWJO	https://www.flowjo.com/solutions/flowjo/downloads
Motioncor2	Zheng et al., 2017	N/A
COOT	Emsley and Cowtan, 2004	https://www2.mrc-lmb.cam.ac.uk/personal/peemsley/coot/
Phenix	Adams et al., 2010	http://www.phenix-online.org/
MolProbity		N/A