Fig. 5. Light-induced conversion from specific to nonspecific Rad4–DNA complexes as tracked by FLT. (A) FLT distributions obtained from MEM analyses of Rad4-bound NPOM-DNA (AT7_DA) irradiated with varying photocleavage times (0–240 s). (B) FLT distributions of NPOM-DNA with varying DNA:Rad4 ratios. (C) Overlay of 30 s irradiation and 1 : 2 AT7_DA : Rad4 complex. (D) Overlay of 60 s irradiation and 1 : 3 AT7_DA : Rad4 complex.
