Figure 7.

Blocking NMDA receptors does not prevent the emergence of slow oscillatory calcium activity in Pyr:Kcnq2 slices. All recordings were in the presence of 8 mm Ko. A, Representative examples of control and Pyr:Kcnq2 2D plots show calcium activity across ROIs and the corresponding wavelets. 2D plots and wavelets before and after application of 25 μm D-APV (APV) are shown. Note that APV reduced the power of the slow oscillatory activity in Pyr:Kcnq2 slices. B, Comparison of the probability density for the transient oscillation frequency ($f$) in the presence and absence of APV in control (n = 20) and Pyr:Kcnq2 (n = 14) hemispheres. Note that APV did not change the likelihood of the emergence of a slow oscillatory activity at the 0.03- to 0.05-Hz frequency range. C, Summary graphs show the effect of APV in control and Pyr:Kcnq2 hemispheres on the power at the LF (0.02–0.2 Hz) and HF (0.2–2 Hz) domains. Note that APV reduced the power across all oscillatory frequencies, suggesting that NMDA receptors promote the calcium activity. D, Summary graphs show the effect of APV on the number of ROIs in control and Pyr:Kcnq2 slices, demonstrating sustained oscillations at frequency f*. Note that blocking NMDA receptors led to a decrease in the number of ROIs undergoing sustained oscillations in Pyr:Kcnq2 hemispheres, consistent with our observed reduction in power shown in C. Data are represented as mean ± SEM (**p < 0.01, ***p < 0.001, ****p < 0.0001). Additional details on the statistical analysis for panel C are found in Table 1 under the Figure 7 section. nd, not determined.