Baseline |
78 ± 9 |
|
|
Superficial |
Deep |
|
|
Superficial |
Deep |
E + 20 |
26 ± 3 |
|
Baseline |
1.80 ± 0.61 |
1.24 ± 0.31 |
|
Sham (spike/s) |
5.76 ± 4.71 |
6.44 ± 4.22 |
E + 120 |
34 ± 3 |
|
E + 20 |
0.16 ± 0.04 |
0.26 ± 0.06 |
|
Eunucleated |
4.56 ± 3.63 |
6.9 ± 4.91 |
|
|
|
E + 60 |
0.42 ± 0.19 |
0.48 ± 0.08 |
|
Sham (Cont.) |
0.73 ± 0.22 |
0.87 ± 0.14 |
|
|
|
E + 120 |
0.50 ± 0.29 |
0.53 ± 0.18 |
|
Enucleated |
0.64 ± 0.25 |
0.78 ± 0.17 |
|
|
|
|
|
|
|
|
|
|
Figure 3B |
log10(spikes per second) |
|
|
|
|
|
|
|
|
Superficial |
P8–P9 |
P10 |
P11 |
P12 |
P13 |
P14 |
P15 |
P16–P17 |
ANOVA |
Sham |
−0.43 ± 0.49 |
−0.03 ± 0.34 |
0.16 ± 0.40 |
0.25 ± 0.19 |
0.29 ± 0.22 |
0.66 ± 0.26 |
0.76 ± 0.22 |
0.87 ± 0.13 |
Age F = 63.5 p < 10−10
|
Enucleated |
−0.37 ± 0.36 |
−0.18 ± 0.16 |
−0.10 ± 0.05 |
0.40 ± 0.14 |
0.52 ± 0.19 |
0.49 ± 0.19 |
0.73 ± 0.24 |
1.07 ± 0.10 |
Group F = 1.18 p = 0.30 |
|
|
|
|
|
|
|
|
|
Layer F = 1.57 p = 0.21 |
Deep |
|
|
|
|
|
|
|
|
A × G F = 2.03 p = 0.06 |
Sham |
−0.51 ± 0.29 |
−0.02 ± 0.25 |
0.24 ± 0.33 |
0.33 ± 0.26 |
0.55 ± 0.44 |
0.87 ± 0.18 |
0.10 ± 0.07 |
0.95 ± 0.18 |
A × L F = 0.96 p = 0.47 |
Enucleated |
−0.53 ± 0.32 |
−0.12 ± 0.29 |
−0.06 ± 0.15 |
0.40 ± 0.40 |
0.76 ± 0.32 |
0.61 ± 0.23 |
0.61 ± 0.20 |
0.90 ± 0.09 |
G × L F = 1.63 p = 0.20 |
|
|
|
|
|
|
|
|
|
A × G × L F = 0.26 p = 0.97 |
|
|
|
|
|
|
|
|
|
|
Figure 3C |
Continuity |
|
|
|
|
|
|
|
|
Superficial |
P8–P9 |
P10 |
P11 |
P12 |
P13 |
P14 |
P15 |
P16–P17 |
|
Sham |
0.11 ± 0.06 |
0.19 ± 0.05 |
0.24 ± 0.13 |
0.40 ± 0.20 |
0.47 ± 0.12 |
0.77 ± 0.19 |
0.78 ± 0.15 |
0.74 ± 0.19 |
Age F = 61.47 p < 10−10
|
Enucleated |
0.09 ± 0.01 |
0.13 ± 0.04 |
0.17 ± 0.04 |
0.45 ± 0.11 |
0.41 ± 0.05 |
0.36 ± 0.09 |
0.41 ± 0.16 |
0.70 ± 0.11 |
Group F = 12.85 p = 0.0005 |
|
|
|
|
|
|
|
|
|
Layer F = 39.21 p = 10−8
|
Deep |
|
|
|
|
|
|
|
|
A × G F = 6.97 p = 10−6
|
Sham |
0.13 ± 0.07 |
0.29 ± 0.12 |
0.32 ± 0.08 |
0.45 ± 0.11 |
0.72 ± 0.28 |
0.94 ± 0.07 |
0.96 ± 0.04 |
0.87 ± 0.16 |
A × L F = 3.24 p = 0.0035 |
Enucleated |
0.09 ± 0.05 |
0.25 ± 0.16 |
0.24 ± 0.10 |
0.52 ± 0.16 |
0.92 ± 0.11 |
0.65 ± 0.25 |
0.67 ± 0.22 |
0.87 ± 0.08 |
G × L F = 1.71 p = 0.19 |
|
|
|
|
|
|
|
|
|
A × G × L F = 0.44 p = 0.88 |
|
|
|
|
|
|
|
|
|
|
Figure 4B |
Distance vs P16–P17 distribution |
|
|
|
|
|
|
|
|
Superficial |
P8–P9 |
P10–P11 |
|
P12 |
P13 |
P14 |
P15 |
P16–P17 |
|
Sham |
0.57 ± 0.05 |
0.49 ± 0.02 |
|
0.51 ± 0.05 |
0.43 ± 0.03 |
0.45 ± 0.04 |
0.29 ± 0.02 |
0.27 ± 0.09 |
Age F = 28.19 p < 10−10
|
Enucleated |
0.62 ± 0.08 |
0.52 ± 0.05 |
|
0.42 ± 0.04 |
0.38 ± 0.03 |
0.37 ± 0.02 |
0.49 ± 0.06 |
0.38 ± 0.08 |
Group F = 6.38 p = 0.0118 |
|
|
|
|
|
|
|
|
|
Layer F = 14.29 p = 0.0002 |
Deep |
|
|
|
|
|
|
|
|
A × G F = 2.20 p = 0.06 |
Sham |
0.79 ± 0.07 |
0.48 ± 0.03 |
|
0.63 ± 0.08 |
0.55 ± 0.06 |
0.52 ± 0.05 |
0.42 ± 0.04 |
0.20 ± 0.09 |
A × L F = 3.11 p = 0.0053 |
Enucleated |
0.82 ± 0.06 |
0.64 ± 0.05 |
|
0.57 ± 0.06 |
0.55 ± 0.06 |
0.52 ± 0.03 |
0.53 ± 0.05 |
0.39 ± 0.08 |
G × L F = 0.48 p = 0.49 |
|
|
|
|
|
|
|
|
|
A × G × L F = 1.17 p = 0.32 |
|
|
|
|
|
|
|
|
|
|
Figure 5B |
Distance vs P16–P17 distribution |
|
|
|
|
|
|
|
|
Superficial |
P8–P9 |
P10–P11 |
|
P12 |
P13 |
P14 |
P15 |
P16–P17 |
|
Sham |
0.68 ± 0.02 |
0.70 ± 0.03 |
|
0.83 ± 0.07 |
0.49 ± 0.03 |
0.35 ± 0.05 |
0.29 ± 0.02 |
0.20 ± 0.07 |
Age F = 69.16 p < 10−10
|
Enucleated |
0.72 ± 0.05 |
0.77 ± 0.07 |
|
0.36 ± 0.03 |
0.41 ± 0.05 |
0.41 ± 0.03 |
0.48 ± 0.09 |
0.32 ± 0.09 |
Group F = 11.06 p = 0.0009 |
|
|
|
|
|
|
|
|
|
Layer F = 2.28 p = 0.13 |
Deep |
|
|
|
|
|
|
|
|
A × G F = 13.28 p < 10−10
|
Sham |
0.78 ± 0.04 |
0.64 ± 0.04 |
|
0.75 ± 0.07 |
0.51 ± 0.04 |
0.36 ± 0.04 |
0.29 ± 0.03 |
0.29 ± 0.07 |
A × L F = 1.98 p = 0.067 |
Enucleated |
0.91 ± 0.05 |
0.88 ± 0.06 |
|
0.48 ± 0.05 |
0.21 ± 0.03 |
0.42 ± 0.04 |
0.49 ± 0.07 |
0.33 ± 0.09 |
G × L F = 0.07 p = 0.79 |
|
|
|
|
|
|
|
|
|
A × G × L F = 2.60 p = 0.017 |