KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti ASCL1 | BD Biosciences | Cat # 556604 Clone 24B72D11.1 |
| Rabbit monoclonal anti NEUROD1 | Abcam | Cat # ab213725 Clone EPR20766 |
| Rabbit polyclonal anti POU2F3 | Novus Biologicals | Cat # NBP1–83966 |
| Rabbit polyclonal anti vimentin | Cell Signaling Technologies | Cat # 5741 Clone D21H3 |
| Goat polyclonal anti AXL | R&D Systems | Cat# AF154 |
| Rabbit monoclonal anti BCL2 | Abcam | Cat# ab32124 Clone E17 |
| Rabbit polyclonal anti vimentin | Cell Signaling Technologies | Cat # 3932 |
| Rabbit monoclonal anti E-Cadherin | Cell Signaling Technologies | Cat # 3195 Clone 24E10 |
| Goat polyclonal anti actin | Santa Cruz | Cat # sc-1616 |
| Mouse monoclonal anti SSTR2 Alexa Fluor 594 conjugated | Novus Biological | Cat # IC4224T Clone 402038 |
| Mouse monoclonal anti BCL2 | Dako | Cat # M0887 Clone 124 |
| Mouse monoclonal anti AXL | Abcam | Cat # ab54803 |
| Rabbit polyclonal anti DLL3 | Cell Signaling Technologies | Cat # 2483 |
| Rabbit monoclonal anti cMYC | Abcam | Cat # ab32072 Clone Y69 |
| Rabbit monoclonal anti TTF1 | Epitomics | Cat # 5883–1 Clone EPR8190 |
| Rabbit polyclonal anti P53 | Cell Signaling Technologies | Cat # 9282 |
| Mouse monoclonal anti RB1 | Cell Signaling Technologies | Cat # 9309 Clone 4H1 |
| Polyclonal rabbit anti E-Cadherin | Cell Signaling Technologies | Cat # 4065 |
| Bacterial and Virus Strains | ||
| None | ||
| Biological Samples | ||
| Circulating tumor cell-derived xenografts (CDX) | MD Anderson Cancer Center (Stewart et al., 2020) |
MDA-SC16 MDA-SC49 MDA-SC53 MDA-SC68 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 5-aza-2’-deoxycytidine | Millipore Sigma | Cat # 189825 |
| GSK-LSD1 inhibitor | Millpore Sigma | Cat # SML1072 |
| Mocetinostat | Selleck Chemicals | Cat # S1122 |
| Recombinant TGFβ | Millipore Sigma | Cat # GF111 |
| Cisplatin | MD Anderson Pharmacy | |
| Critical Commercial Assays | ||
| None | ||
| Deposited Data | ||
| SCLC tumor transcriptomic data | George et al., 2015 | EGAS00001000925 |
| SCLC tumor transcriptomic data | Sato et al., 2013 | GSE99312 |
| SCLC cell line transcriptomic data | Polley et al., 2016 | GSE73162 |
| SCLC cell line methylation data | Polley et al., 2016 | GSE145156 |
| SCLC cell line transcriptomic data | This paper | GSE151904 |
| SCLC cell line drug response data | Polley et al., 2016 | https://sclccelllines.cancer.gov/sclc/ |
| SCLC cell line drug response data | Stewart et al., 2017 | N/A |
| SCLC CDX single-cell RNAseq | Stewart et al., 2020 | GSE138474 |
| IMpower133 tumor transcriptomic data | This paper | EGAS00001004888 |
| Experimental Models: Cell Lines | ||
| Human: H446 cells | ATCC | Cat # HTB-171 |
| Human: H1048 cells | ATCC | Cat # CRL-5853 |
| Human: H847 cells | ATCC | Cat # CRL-5846 |
| Human: H211 cells | ATCC | Cat # CRL-5824 |
| Human: H196 cells | ATCC | Cat # CRL-5823 |
| Human: H526 cells | ATCC | Cat # CRL-5811 |
| Human: H841 cells | ATCC | Cat # CRL-5845 |
| Human: H82 cells | ATCC | Cat # HTB-175 |
| Human: DMS79 cells | ATCC | Cat # CRL-2049 |
| Human: H1930 cells | ATCC | Cat # CRL-5906 |
| Human: H341 cells | ATCC | Cat # CRL-5864 |
| Human: H146 cells | ATCC | Cat # HTB-173 |
| Human: H250 cells | ATCC | Cat # CRL-5828 |
| Human: H510A cells | ATCC | Cat # HTB-184 |
| Human: DMS273 cells | Sigma Aldrich | Cat # 95062830–1VL |
| Human: H69 cells | ATCC | Cat # HTB-119 |
| Human: H524 cells | ATCC | Cat # CRL-5831 |
| Human: H889 cells | ATCC | Cat # CRL-5817 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Athymic Nude-Foxn1nu | Envigo | Cat# 069-US |
| Oligonucleotides | ||
| qRT-PCR primers for ASCL1 | BioRad | qHsaCED0007040 |
| qRT-PCR primers for NEUROD1 | BioRad | qHsaCID0006095 |
| qRT-PCR primers for GAPDH | BioRad | pHsaCED0038674 |
| qRT-PCR primers for RPL13A | BioRad | qHsaCED0020417 |
| Recombinant DNA | ||
| None | ||
| Software and Algorithms | ||
| FlowJo version 10.7.1 | BD | www.flowjo.com |
| CIBERSORT | Stanford University | www.cibersort.stanford.edu/ |
| R versions 3.3.0; 3.5.1; 3.6.3; 4.0.1 |
R Foundation for Statistical Computing | www.r-project.org |
| Bioconductor | www.bioconductor.org | |
| Python version 3.7.6 | Python Software Foundation | www.python.org |
| Other | ||
| None | ||