Table 4.
List of six polymorphic KAPS primers validated for resistance to MLN using 20 parent lines including a resistant donor parent.
| Assay code | Primer name | Primer sequence | Remarks |
|---|---|---|---|
| B0051_FAm | S3_44062810_FAm | gaaggtgaccaagttcatgctATCCGCCTTATTGCCGGg | |
| B0051_HEX | S3_44062810_HEX | gaaggtcggagtcaacggattATCCGCCTTATTGCCGGa | Polymorphic, linked to QTL with minor effects |
| B0051_COm | S3_44062810_COm | AGGATTAACGACGGGAAGGT | |
| B0054_FAm | S3_146966676_FAm | gaaggtgaccaagttcatgctGTCCTGCTGCTGGAGCGt | |
| B0054_HEX | S3_146966676_HEX | gaaggtcggagtcaacggattGTCCTGCTGCTGGAGCGc | Polymorphic, linked to QTL with minor effects |
| B0054_COm | S3_146966676_COm | GTAGGCGTCCCGGATGAT | |
| B0056_FAm | S3_146250249_FAm | gaaggtgaccaagttcatgctCTACCCATCCGCCTGCTt | |
| B0056_HEX | S3_146250249_HEX | gaaggtcggagtcaacggattCTACCCATCCGCCTGCTg | Polymorphic, linked to QTL with major effects |
| B0056_COm | S3_146250249_COm | CACCTGGCACGGAGAGAAG | |
| B0057_FAm | S3_146363360_FAm | gaaggtgaccaagttcatgctACCAGGACAGGTATCTAACGCc | |
| B0057_HEX | S3_146363360_HEX | gaaggtcggagtcaacggattACCAGGACAGGTATCTAACGCt | Polymorphic, linked to QTL with major effects |
| B0057_COm | S3_146363360_COm | CGTACCAGGTCTGAGCACAA | |
| B0060_FAm | S6_21007530_FAm | gaaggtgaccaagttcatgctGCAAAAATCACAGCCGATCg | |
| B0060_HEX | S6_21007530_HEX | gaaggtcggagtcaacggattGCAAAAATCACAGCCGATCa | Polymorphic, linked to QTL with minor effects |
| B0060_COm | S6_21007530_COm | CCGGGCCTAAAGCCTAATAC | |
| B0065_FAm | S6_157568432_FAm | gaaggtgaccaagttcatgctGCATAGAAATAAAATGAGACAAGGg | |
| B0065_HEX | S6_157568432_HEX | gaaggtcggagtcaacggattGCATAGAAATAAAATGAGACAAGGt | Polymorphic, linked to QTL with minor effects |
| B0065_COm | S6_157568432_COm | ATCCATGTTGTCCCTCCGTA |