TABLE 4.

Experimental designs supported by different open-source programs used for mapping-by-sequencing.

Mutations under study	Mapping design	Control sample	Software
			SHOREmap1 artMAP2	SIMPLE3	CandiSNP4	Jitterbug5 ITIS6	Easymap7
Point mutations	Backcross	Parental line	D		D		D/R
		Phenotypically wild-type F2 or M2		D			D/R
	Outcross	Parental line	D				D/R
		Phenotypically wild-type F2 or M2		D			D/R
Large insertions	–	–				D	D

The capabilities of some current mapping tools are compared, D and R indicating an experimental design supported by the software (with DNA-seq or RNA-seq data, respectively). ¹Sun and Schneeberger (2015). ²Javorka et al. (2019). ³Wachsman et al. (2017). ⁴Etherington et al. (2014). ⁵Hénaff et al. (2015). ⁶Jiang et al. (2015). ⁷This work.