Figure 4.

Age reversal precedes reversion of developmental state during reprogramming of fibroblasts using OKSM germline factors. Post-EFT genes (COX7A1, ADIRF) are rapidly reduced during early (partial) reprogramming (day 3–20) while a linear decrease in DNAm age is occurring. Pre-EFT genes are turned on at day 20 when DNAm age has gone to zero, and their expression increases as cells reverse their developmental state to full pluripotency (≥28 days). The data suggest the possibility of age reprogramming without permanent loss of cell identity. Figure is adapted from Olova et al. Figure 1 [162].