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. 2021 Apr 21;10(5):349. doi: 10.3390/biology10050349

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Alteration in mitophagy and autophagy markers in remnant mass. (A) Western blots and their quantifications of mitophagy proteins: phosphate and tensin homolog (PTEN)-induced kinase 1 (PINK1) and PARKIN in isolated mitochondria. Voltage-dependent anion channel (VDAC) was used as loading control. (B) Western blots and their quantifications of autophagy proteins: p62 and microtubule-associated proteins 1A/1B light chain 3B I and II (LC3B-I/II). β-Actin was used as loading control. Data are the mean ± SEM, n = 4. Tukey test. a = p ≤ 0.05 vs. sham, b = p ≤ 0.05 vs. 2 days, c = p ≤ 0.05 vs. 4 days, d = p ≤ 0.05 vs. 7 days, e = p ≤ 0.05 vs. 14 days, 5/6Nx = 5/6 nephrectomy, d = days after 5/6Nx, sham = simulated operation/control group.