Table 1.
Student's t test and one- or two-way ANOVA with their respective post-hoc comparisons for each figure
| Figure | Test | Post hoc comparison |
|---|---|---|
| 1B | Ordinary one-way ANOVA | Dunnett's test |
| Males: F(6,35) = 35.60, p < 0.0001 | Baseline × Fracture at 3 weeks: p < 0.0001, at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001, at 9 weeks: p < 0.0001, at 12 weeks: p < 0.0001, at 20 weeks: p = 0.0067 | |
| Females: F(6,35) = 32.99, p < 0.0001 | Baseline × Fracture at 3 weeks: p < 0.0001, at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001, at 9 weeks: p < 0.0001, at 12 weeks: p < 0.0001, at 20 weeks: p = 0.0315 | |
| 1C | Paired t test | NA |
| Males: t = 12.24, p = 0.0012 | ||
| Females: t = 12.69, p = 0.0011 | ||
| 1D | Unpaired t test | NA |
| Males: t = 8.481, p < 0.0001 | ||
| Females: t = 14.53, p < 0.0001 | ||
| 1E | Unpaired t test | NA |
| Males: t = 8.436, p < 0.0001 | ||
| Females: t = 18.35, p < 0.0001 | ||
| 2C | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(6,58) = 4.975, p = 0.0004 | Vehicle × Early TAK at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001 | |
| Time effect: F(3,58) = 134.1, p < 0.0001 | Vehicle × Late TAK at 5 weeks: p < 0.0001, at 7 weeks: p = 0.0376 | |
| Treatment effect: F(2,58) = 17.51, p < 0.0001 | Early TAK × Late TAK at 7 weeks: p = 0.0121 | |
| 2D | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(6,57) = 8.173, p < 0.0001 | Vehicle × Early TAK at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001 | |
| Time effect: F(3,57) = 113.3, p < 0.0001 | Vehicle × Late TAK at 5 weeks: p < 0.0001 | |
| Treatment effect: F(2,57) = 13.26, p < 0.0001 | Early TAK × Late TAK at 7 weeks: p = 0.0002 | |
| 2E | Ordinary one-way ANOVA | Tukey's test |
| Males: F(2,19) = 6.212, p = 0.0084 | Vehicle × Treatment for early TAK: p = 0.0141, for late TAK: p = 0.0396 | |
| 2F | Ordinary one-way ANOVA | Tukey's test |
| Females: F(2,19) = 10.94, p < 0.0001 | Vehicle × Treatment for late TAK: p = 0.0005 | |
| 2G | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(18,252) = 2.568, p = 0.0006 | Male vehicle × Male LPS-rs at 1 h: p = 0.0253, at 2 h: p = 0.0002, at 4 h: p = 0.0001 | |
| Time effect: F(6,252) = 195.4, p < 0.0001 | Male LPS-rs × Female LPS-rs at 1 h: p = 0.0474, at 2 h: p = 0.0026, at 4 h: p = 0.0001 | |
| Treatment effect: F(3,252) = 9.219, p < 0.0001 | ||
| 2H | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(9,144) = 8.462, p < 0.0001 | Female vehicle × Female RS504393 at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001 | |
| Time effect: F(3,144) = 221.4, p < 0.0001 | Male RS504393 × Female RS504393 at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001 | |
| Treatment effect: F(3,144) = 26.24, p < 0.0001 | ||
| 3F | Ordinary one-way ANOVA | Tukey's test |
| CNS: F(2,9) = 19.31, p = 0.0006 | Control × TLR4 cKO at 7 d: p = 0.0005, at 28 d: p = 0.0054 | |
| 3G | Ordinary one-way ANOVA | Tukey's test |
| Spleen: F(2,7) = 5.199, p = 0.0413 | Control × TLR4 cKO at 7 d: p = 0.0944, at 28 d: p = 0.9920 | |
| 3H | Ordinary one-way ANOVA | Tukey's test |
| Microglia: F(2,3) = 1108, p < 0.0001 | Control × TLR4 cKO in male: p < 0.0001, in female: p < 0.0001 | |
| 4B | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(18,147) = 10.47, p < 0.0001 | Male control × Male early TLR4 cKO at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001, at 9 weeks: p < 0.0001, at 12 weeks: p = 0.0105 | |
| Time effect: F(6,147) = 135.7, p < 0.0001 | Female control × Female early TLR4 cKO at 7 weeks: p = 0.0010, at 9 weeks: p = 0.0125, at 12 weeks: p = 0.0009 | |
| Genotype effect: F(3,147) = 48.95, p < 0.0001 | Male early TLR4 cKO × Female early TLR4 cKO at 5 weeks: p < 0.0001, at 7 weeks: p < 0.0001, at 9 weeks: p < 0.0001 | |
| 4C | Unpaired t test | NA |
| Males: t = 4.539, p = 0.0027 | ||
| 4D | Unpaired t test | NA |
| Females: t = 0.6142, p = 0.5561 | ||
| 4H | Unpaired t test | NA |
| Males: t = 2.307, p = 0.0499 | ||
| 4J | Unpaired t test | NA |
| Males: t = 0.8198, p = 0.4361 | ||
| 4N | Unpaired t test | NA |
| Females: t = 0.3177, p = 0.7589 | ||
| 4P | Unpaired t test | NA |
| Females: t = 0.8877, p = 0.4006 | ||
| 5B | Unpaired t test | NA |
| Males: t = 3.825, p = 0.0065 | ||
| 5D | Unpaired t test: Lamina I | NA |
| Males: t = 3.530, p = 0.0096 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 3.131, p = 0.0166 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 3.633, p = 0.0084 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 3.676, p = 0.0079 | ||
| 5E | Unpaired t test | NA |
| Males: t = 2.768, p = 0.0278 | ||
| 5G | Unpaired t test: Lamina I | NA |
| Males: t = 1.019, p = 0.3420 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 2.673, p = 0.0319 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 1.908, p = 0.0980 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 1.707, p = 0.1316 | ||
| 6B | Unpaired t test | NA |
| Males: t = 0.7979, p = 0.4480 | ||
| 6C | Unpaired t test: Lamina I | NA |
| Males: t = 0.1327, p = 0.8977 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 0.5364, p = 0.6062 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 1.041, p = 0.3281 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 1.126, p = 0.2928 | ||
| 6D | Unpaired t test | NA |
| Males: t = 1.066, p = 0.3174 | ||
| 6E | Unpaired t test: Lamina I | NA |
| Males: t = 0.5622, p = 0.5894 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 0.7495, p = 0.4750 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 0.8380, p = 0.4263 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 1.326, p = 0.2214 | ||
| 7B | Two-way ANOVA: | Bonferroni's test |
| Interaction: F(18,184) = 3.238, p < 0.0001 | Male control × Male late TLR4 cKO at 5 weeks: p = 0.0197, at 7 weeks: p = 0.0007, at 9 weeks: p = 0.0033 | |
| Time effect: F(6,184) = 138.3, p < 0.0001 | Female control × Female late TLR4 cKO at 5 weeks: p = 0.0004, at 12 weeks: p = 0.0085 | |
| Genotype effect: F(3,184) = 13.22, p < 0.0001 | ||
| 7C | Unpaired t test | NA |
| Males: t = 2.955, p = 0.0131 | ||
| 7D | Unpaired t test | NA |
| Females: t = 4.735, p = 0.0011 | ||
| 7H | Unpaired t test | NA |
| Males: t = 1.180, p = 0.2630 | ||
| 7J | Unpaired t test | NA |
| Males: t = 1.282, p = 0.2261 | ||
| 7N | Unpaired t test | NA |
| Females: t = 1.309, p = 0.2228 | ||
| 7P | Unpaired t test | NA |
| Females: t = 1.546, p = 0.1564 | ||
| 8B | Unpaired t test | NA |
| Males: t = 0.8275, p = 0.4319 | ||
| 8C | Unpaired t test: Lamina I | NA |
| Males: t = 2.968, p = 0.0179 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 1.338, p = 0.2176 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 0.1216, p = 0.9062 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 0.2830, p = 0.7844 | ||
| 8D | Unpaired t test | NA |
| Males: t = 4.766, p = 0.0014 | ||
| 8E | Unpaired t test: Lamina I | NA |
| Males: t = 1.657, p = 0.1360 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 5.182, p = 0.0008 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 4.453, p = 0.0021 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 1.266, p = 0.2411 | ||
| 9B | Unpaired t test | NA |
| Males: t = 0.5207, p = 0.6152 | ||
| 9C | Unpaired t test: Lamina I | NA |
| Males: t = 2.753, p = 0.0224 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 0.7424, p = 0.4768 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 0.3539, p = 0.7316 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 0.9197, p = 0.3817 | ||
| 9D | Unpaired t test | NA |
| Males: t = 4.058, p = 0.0028 | ||
| 9E | Unpaired t test: Lamina I | NA |
| Males: t = 5.975, p = 0.0002 | ||
| Unpaired t test: Lamina II | NA | |
| Males: t = 4.118, p = 0.0026 | ||
| Unpaired t test: Lamina III | NA | |
| Males: t = 2.921, p = 0.0170 | ||
| Unpaired t test: Lamina IV | NA | |
| Males: t = 1.676, p = 0.1281 |