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. 2021 May 12;41(19):4187–4201. doi: 10.1523/JNEUROSCI.2631-20.2021

Figure 2.

Figure 2.

Synapsin is required for cAMP-induced neuropeptide release. a, NLP-21::Venus neuropeptides are released from cholinergic neurons and endocytosed by coelomocytes. b, Representative images of coelomocytes in bPAC, snn-1, and snn-1;bPAC animals, without and with photostimulation. False color representation: scale bar, 10 µm. c, Fluorescence quantification in coelomocytes, normalized to fluorescence level in nonstimulated wt. Data are median and 25/75 quartiles (thick and thin lines), min to max. Fluorescence (d) and quantification (e) of NLP-21::Venus in cell bodies and processes of cholinergic neurons, in wt and snn-1(tm2557) mutants. Number of animals indicated. c, e, Data are mean ± SEM. **p < 0.01; ***p < 0.001; two-way or one-way ANOVA with Tukey test or Bonferroni correction.