Figure 1.

Wide-field calcium imaging of cortex-wide activity. A, Left, Imaging setup. Middle, An FOV of wide-field calcium imaging in a mouse cortex expressing GCaMP6s in cortical excitatory neurons. Right, Cortical regions (based on the mouse brain atlas from the Allen Institute) simultaneously recorded by wide-field calcium imaging. Green dashed box represents the area within the FOV. M2, Secondary motor cortex; M1, primary motor cortex; S1, primary somatosensory cortex; PPC, posterior parietal cortex; S2, secondary somatosensory cortex; Aud., auditory cortex; RSC, retrosplenial cortex; Vis., visual cortex. B, Example cortex-wide image frames and fluorescence traces of individual pixels in a behaving mouse. Gray dashed lines indicate the time of image frames in fluorescence traces of example pixels.