Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 14;17(5):1259–1277. doi: 10.1080/15548627.2020.1816664

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Transcriptional repression of the MAP1LC3 genes is observed upon autophagy induction. (A) MAP1LC3B and MAP1LC3B2 isoforms mRNA expression measured by RT-qPCR in previously autophagy-exposed U1810, HeLa and MEF cells processed as described in Fig. S1A and analyzed after a specific recovery period. (B) Analysis of mRNA expression of 84 autophagy-related genes (from a RT-qPCR based gene array) in HeLa cells following a 3 weeks recovery-period after an initial torin1 or DMSO (used as control) treatment for 4 h. Representation of n = 3 independent experiments. (C) Analysis by RT-qPCR of mRNA expression of ATG10, ATG13, PIK3C3, RB1CC1, ULK1, GAPARAPL1, GABARAPL2 and GABARAP genes in HeLa cells processed as described in panel B. All values are means of at least 3 independent experiments ± SEM and considered significant for *p < 0.05, **p < 0.01, ***p < 0.001. n.s., not significant for the indicated comparison. (A, n = 4–6; B and C, n = 3)