Figure 1.

Cre recombinase efficiently targets conventional DCs in cybb^fl/fl-Itgax-Cre animals. (A) Representative histograms depicting Cre-GFP expression of either ITGAX^hi MHCII^hi splenic DCs (cDCs), LY6C1^hi ITGAM⁺ monocytes (LY6C1^hi Monos), LY6C1^lo ITGAM⁺ monocytes (LY6C1^lo Monos) or CD19⁺ MHCII⁺ B cells in Cybb^fl/fl (gray) or cybb^fl/fl-Itgax-Cre (red) mice in steady-state (upper panel). Quantification of Cre-GFP median fluorescence intensity (MFI) in splenic leukocyte subsets. Each data point represents one individual animal (middle panel). Western blot analysis for protein expression of CYBB in the respective cellular compartments is shown. ACTB served as a loading control (lower panel). Statistical analysis: Unpaired two-tailed Student t-test was applied. Mean ± SEM is depicted. ns, not significant: P > 0.05; ***P < 0.001. (B) Quantification of MFI for Cre-GFP, MHCII, CD80, and CD86 in CNS-resident immune cell subsets. Each data point represents one individual animal. One representative out of 3 experiments is shown. Statistical analysis: Unpaired two-tailed Student t-test was applied. Mean ± SEM is depicted. ns, not significant: P > 0.05; *P < 0.05; ***P < 0.001. cDC, conventional dendritic cells; BAMS, border-associated macrophages; iB cells, immature B cells