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Immunohistochemical analysis shows CTSD localization in the larval midgut during metamorphosis. Rabbit polyclonal antibodies against CTSD were used. The preserum was used as the negative control. LM: larval midgut; IM: imaginal midgut; hematoxylin and eosin (HE) staining showed the morphology of the midgut; Red fluorescence indicates CTSD; Nuclei were stained with DAPI (blue). Scale bar: 50 μm
