Figure 4.

H₂O₂ accumulation in ccoaomt1 mutants by drought stress. (A) DAB staining of drought-stressed leaves. The leaves of 10-day-old seedlings of WT and ccoaomt1 mutants were stained with 3,3′-Diaminobenzidine (DAB) before and after drought stress (for 60 min). The red color represents insoluble ROS products, which hydrogen peroxide (H₂O₂) reacts with DAB. (B) Quantified H₂O₂ content. Internal H₂O₂ production assays were performed using WT and ccoaomt1 mutants and the fluorescence intensity was measured with the range excitation at 530 nm and emission at 590 nm. The quantitative values indicated means ± SD of three independent experiments. Asterisks represent significant differences from the WT (*, p < 0.05; **, p < 0.01; Student’s t-test).