Figure 4.

Alignment of crustacyanin amino acid sequences. Unigene0054180-EcR and Unigene0054180-EcW represented the crustacyanin in the orange-red-variant prawns and the wild-type prawns. A1 and A3 represented the crustacyanin subunit proteins in the lobster. Amino acid sequences of lipocalin consensus regions were colored, and different amino acid residues between the orange-red-variant prawns and the wild-type prawns were highlighted in the red box. Asterisks (*) indicated positions which had a single, fully conserved residue, while one black dot (.) and two black dots (:) indicated that the residue sites were less conserved but their physicochemical properties were similar or very similar.