Table 1.
Information of primers used for real-time PCR.
| Gene ID | Description | Sequence (5′ to 3′) | Tm (°C) |
|---|---|---|---|
| Unigene0059050 | Esterase FE4 | F: GTTGGACCAAATTTCGGCTCTT R: TGGAGAGAGCGCATGAAAGTTA |
59 |
| Unigene0054180 | Crustacyanin subunit A | F: TCCTGAGGGAACGCACGAGA R: TGGAGAAGACGAAGGCGAACT |
58 |
| Unigene0036561 | Apolipoprotein D | F: CTGGGCACAGATTACGAGAACT R: GGTGGGTACGATGGAAGAGG |
64 |
| Unigene0037069 | Cuticular protein 34 | F: CAGAAGCCACGAGCCAGAAG R: GGGTCCTACGAGGATGATGTTAT |
57 |
| Unigene0038920 | Cuticle proprotein | F: CGTAACTGGCACCTGGATGA R: AGAGGATAGCGTGGGCTGGA |
59 |
| Unigene0043447 | Cuticular protein 34 | F: TCGTCAGAGCCGATGGAAAC R: TGGGAGTGCCATCATCAGTG |
58 |
| Unigene0030347 | None | F: CTATGCGAATGAATAAGATGAGGAG R: CCAGCGTACCAAGTAATACTGAAA |
57 |
| Unigene0033842 | Retrovirus-related Pol polyprotein | F: CGATTCACTGTCCCCACTACTC R: GTCTATTTCCTTGATGCTCTTACCA |
56 |
| Unigene0047842 | Lathosterol oxidase | F: AGTGTAAGAATCCACCAATGCC R: GCCAAGTTTCCAATGAGACAGC |
58 |
| 18S rRNA | 18S rRNA | F: TATACGCTAGTGGAGCTGGAA R: GGGGAGGTAGTGACGAAAAAT |
55 |
Note: F—forward primer; R—reverse primer.