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. 2021 Apr 21;13(5):598. doi: 10.3390/pharmaceutics13050598

Figure 6.

Figure 6

Effects of various human esterases, CYPs, and FMOs on IMMH-010 metabolism. (A), Effects of esterases on IMMH-010 metabolism. IMMH-010 (10 µM) was incubated with HLM (0.2 mg/mL) for 15 min at 37 °C in the presence of chemical inhibitors (left). The selective CES1 and CES2 inhibitors were digitonin (100 μM) and telmisartan (50 μM). IMMH-010 (10 µM) was incubated individually with recombinant human CES1, CES2, and AADAC (0.1 mg protein/mL) at 37 °C for 15 min (right). (B), Effects of CYPs and FMOs on IMMH-010 metabolism. IMMH-010 (10 µM) was incubated individually with 50 pmol of recombinant human CYPs and FMOs at 37 °C for 30 min in the presence of an NADPH regenerating system. Data are expressed as mean ± SD.