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Nucleotide primer sequences used in qRT-PCR analysis in S. oryzae adult stage treated with essential oils.
| Technique | Target Gene | Sequences (5′–3′) | Annealing °C |
|---|---|---|---|
| qRT-PCR | β-Actin-F | GACCTCTATGCCAACACAGT | 60 °C |
| β-Actin-R | AGTACTTGCGCTCAGGAGGA | ||
| CL8-F | CATCCGCAAACACAACAAAC | ||
| CL8 -R | TACCTGAAGGGTCCATATGG | ||
| CYP 4Q4-F | CAGTTTGGTGATTCAGATGATG | ||
| CYP 4Q4-R | GCACATCTGGGGACAAACTT | ||
| CL1294 –F | GTCTATGCACCTGGGTCGTT | ||
| CL1294 –R | GTCGGCAGACAAGGAAGACA |
