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. 2021 May 24;16(5):e0252096. doi: 10.1371/journal.pone.0252096

Fig 1. Pipeline of the methodology followed in this study.

Fig 1

Step 1: Datasets with the accession number GSE# were downloaded from the Gene Expression Omnibus (GEO) or were obtained from a previous study [35] and processed for data integration. Step 2. Temporally differentially expressed genes (DEG) from day 7 (D7) to D13 and D16 and differentially methylated regions (DMR) in the trophectoderm (TE) from D7 to D17 were determined between groups. Differentially methylated genes (DMG) were the genes in the corresponding exons of the DMR. Step 3. DEG and DMG were overlapped to determine common genes, which were further analysed (Fig 2). Each step is explained in detail in the methodology section. IVP: embryos produced in vitro. MOET: embryos produced in vivo (after ovarian superovulation).