Table 3. Next-generation sequencing coverages of Hantaan virus (HTNV) from rodents collected in Jeju Island, 2018–2020.
| Site | Sample | IFA for anti-HTNV IgG | Origin | Ct value | HTNV genomes, % coverage | ||
|---|---|---|---|---|---|---|---|
| L segment | M segment | S segment | |||||
| Ora-dong, Jeju-si | Ac19-6 | 1:1,024a | Lung | 30.0 | 81 | 96.6 | 98.1 |
| Ac20-5 | 1:32b | Lung | 23.8 | 58.6 | 99.2 | 98.4 | |
| Ac20-6 | 1:16b | Lung | 31.5 | 41.4 | 97 | 98.4 | |
| Bongseong-ri, Jeju-si | Ac20-30 | 1:256a | Lung | 25.9 | 93.4 | 99.1 | 98.4 |
| Ac20-31 | 1:256a | Lung | 21.8 | 93 | 99 | 98.4 | |
| Ac20-32 | 1:8b | Lung | 27.7 | 91 | 99.1 | 98.4 | |
Ac, Apodemus chejuensis; IFA, indirect immunofluorescence antibody test; IgG, immunoglobulin G; Ct, cycle threshold; L, large; M, medium; S, small.
a; IFA test was performed from sera
b; IFA test was performed from heart fluids.
Genome coverage was calculated by obtaining consensus sequences matching the prototype HTNV 76–118.
The complete genome sequence lengths of HTNV are the L (6,533 nt), M (3,616 nt), and S segments (1,696 nt), respectively.